Fig. 1.
Detection of mRNA expression of large-conductance voltage- and Ca2+-activated K+ (BK) channel α-, β1-, β2-, β3-, and β4-subunits in human detrusor smooth muscle (DSM) whole tissue (A) and freshly isolated cells (B). The expected product sizes were 455 bp for the α-subunit, 258 bp for the β1-subunit, 376 bp for the β2-subunit, 268 bp for the β3-subunit, 168 bp for the β4-subunit, and 268 bp for the housekeeping gene β-actin. Only α-, β1-, and β4-subunits but not β2 and β3 were detected. Results were verified in 12 DSM preparations obtained from 4 patients.