Fig. 10.

Zip14-dependent Ca²⁺ uptake and the effects of divalent metal ions. A: uptake of 150 μM ⁴⁵Ca²⁺ in the absence (None) or presence of 30 μM Fe²⁺ or 30 μM Cd²⁺ in control oocytes (gray bars) and oocytes expressing Zip14 (black bars, n = 9–12) in media containing 1 mM l-ascorbic acid and 100 μM niflumic acid. Two-way ANOVA revealed an interaction (P < 0.001); within Zip14, ^anot significant (unadjusted P = 0.68) and ^bP < 0.001 cf. “None”. B: uptake of 300 μM ⁴⁵Ca²⁺ in the absence (None) or presence of 15 μM Zn²⁺ in control oocytes (gray bars) and oocytes expressing Zip14 (black bars, n = 12–13) in media containing 100 μM niflumic acid. Two-way ANOVA revealed an interaction (P < 0.001).