Fig. 2.

Western blot analysis of membrane fractions from Xenopus oocytes expressing EGFP-Zip14 and DMT1-EGFP by using anti-green fluorescent protein (GFP) antibody. Each lane (numbered at top) was loaded with membrane fractions (∼3 μg protein per lane) isolated from the following: lane 1, control oocytes; lanes 2 and 3, oocytes expressing DMT1-EGFP; lanes 4 and 5, DMT1; lanes 6 and 7, EGFP-Zip14; lanes 8 and 9, Zip14. Intensities of the immunoreactive bands in each lane of the Western blot were normalized by quantity of protein loaded in each lane determined by densitometric analysis of reversible Ponceau staining (40) of the blot (not shown).