Fig. 1.
Initiation of leukocyte rolling in arterioles results in increased solute permeability (Ps). To induce leukocyte rolling in arterioles, mice were treated with TNF-α (500 ng intrascrotally) 4 h before observations. Rat IgG control isotype (R3–34, 100 μg), anti-P-selectin (RB40.34, 30 μg), anti-CD18 (GAME-46, 100 μg), or a combination of anti-P-selectin and CD18 blocking antibodies (Abs) was injected intravenously; leukocyte rolling (A) was quantified 30 min following Ab injection, and 20 min later, Ps (B) was measured in the same vessels. Blockade of CD18 and P-selectin (separately or together) significantly reduced leukocyte rolling in arterioles and significantly reduced Ps. *Significantly different from IgG control group (P < 0.05). For all groups the measurements were made in n = 10–16 arterioles from 3–5 mice.