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Effects of blocking β2-integrin (CD18) on OSM production in neutrophils contacting EC. Neutrophils were preincubated with or without anti-CD18 or IgG isotype for 15 min at which time they were added to IL-1-activated EC present in the upper chamber of transmigration assay in the presence or absence of anti-GM-CSF neutralizing antibody. Data shown are means of four experiments. *P < 0.05 compared with all other groups.
