Fig. 4.

GM-CSF-induced OSM expression in neutrophils in a dose-dependent manner. A: dose-response of GM-CSF on OSM expression in neutrophils. Isolated nuetrophils were incubated with various concentrations of GM-CSF for 1 h at which point were analyzed for OSM expression using RT PCR (*P < 0.01 compared with control nontreated neutrophils; n = 3). B: time course of OSM mRNA expression in GM-CSF-induced neutrophils. Isolated neutrophils were incubated with 5 ng/ml GM-CSF and OSM expression was analyzed for each time point using RT-PCR (*P < 0.01 compared with zero time point; n = 3).