Fig. 7.

Examples of images of cells expressing redistributed nuclear proteins that recovered their normoxic nuclear locations in response to increased oxygen levels even in the presence of cycloheximide. Cells expressing GFP-tagged Hmo1 (A), Cst6 (B), Rfa1 (C), Skn7 (D), Sdc1 (E), and Swi3 (F), respectively, were grown under hypoxic conditions and then shifted to normoxic conditions for 2 h in the presence or absence of cycloheximide (CHX). The images of cells grown under different conditions were captured. A, images of cells grown in air; H, images of cells grown in a hypoxia chamber; H to A, images of cells grown in a hypoxia chamber and then shifted to air for 2 h; H to A + CHX, images of cells grown in a hypoxia chamber and then shifted to air for 2 h in the presence of cycloheximide. The number of cells showing GFP-tagged proteins in the nucleus (N) or cytosol (C) was counted and the percentage was calculated and is plotted. The scale bar represents 2 μm.