Table 3.
Sequences of sense oligonucleotides used in electrophoretic mobility shift assays
| Oligo Name and Location | Sequence | Description |
|---|---|---|
| TTF-1 (+17/+33 bp) | 5′- CTCCACTCAAGCCAATT -3′ | TTF-1 site at +22/+27 bp |
| TTF-1mutant (+17/+33 bp) | 5′- CTCCACACAACCCAATT -3′ | TTF-1 site at +22/+27 bp |
| TTF-1 (+174/+190 bp) | 5′- TCCTCCTCCAGCCGCCG -3′ | TTF-1 site at +179/+184 bp |
| HNF-3 (−19/+5 bp) | 5′- GCCGGGCTAAAACAAACGCGAGGC -3′ | HNF-3 site at −11/−6 bp |
| HNF-3mutant (−19/+5 bp) | 5′- GCCGGGCTACCACTAACGCGAGGC -3′ | HNF-3 site at −11/−6 bp |
| HNF-3 (−140/−120 bp) | 5′- CTTAAAGGTGTTTACCTTGTC -3′ | HNF-3 site at −132/−127 bp |
| HNF-3mutant (−140/−120 bp) | 5′ CTTAAAGGGTAGAACCTTGTC -3′ | HNF-3 site at −132/−127 bp |
| Sp1 (−55/−38 bp) | 5′- TTACGCCCCGCCTCTGGT -3′ | Sp1 site at −50/−42 bp |
| Sp1 consensus | 5′- ATTCGATCGGGGCGGGGCGAG -3′ | consensus Sp1 |
| Sp1mutant | 5′- ATTCGATCGGTTCGGGGCGAGC -3′ | mutant Sp1 |
| ZBP-1 (−243/−209 bp) | 5′- AAATGGGGGTGGGGGTGTCCAAGGGAGGGGGGAA -3′ | ZBP-89 site at −219/−214 bp |
| ZBP-1mutant (−243/−209 bp) | 5′- AAATGGGGTATTGGGTGTCCAAGTTCTGGGGGAA -3′ | mutant ZBP-89 site at −219/−214 bp |
| ZBP-2 (−279/−262 bp) | 5′- CCACCTCCTCCCCGCGCT -3′ | ZBP-89 site at −271/−266 bp |
| ZBP-2mutant (−279/−262 bp) | 5′- CCACCTCCTAACCGCGCT -3′ | mutant ZBP-89 site at −271/−266 bp |
| ZBP-3 (−293/−276 bp) | 5′- CCTTCCCCTCCCCTCCAC -3′ | ZBP-89 site at −285/−280 bp |
| ZBP-3mutant (−293/−276 bp) | 5′- CCTTCCCCTAACCTCCAC -3′ | mutant ZBP-89 site at −285/−280 bp |
| ZBP-4 (−293/−276 bp) | 5′- CCTTCCCCTCCCCTCCAC -3′ | ZBP-89 site at −297/−292 bp |
| ZBP-4mutant (−293/−276 bp) | 5′- CCTTCCCCTAACCTCCAC -3′ | mutant ZBP-89 site at −297/−292 bp |
| ZBP-5 (−339/−304 bp) | 5′- ACTCCCCCACCCCACCTCCTGCCCTCCCCGCTCTCG -3′ | ZBP site at −315/−310 bp |
| ZBP-5mutant (−339/−304 bp) | 5′- ACTCCCAATACAATACTCCTGCCAGAACCGCTCTCG -3′ | mutant ZBP site at −315/−310 bp |
| ZBP-89p21 | 5′- GAGGGACTGGGGGAGGAGGGAAGTGCCCTC -3′ | ZBP-89 site in p21 promoter |
| TTF-1 SP-B | 5′- GCACCTGGAGGGCTCTTCAGAGCAA -3′ | TTF-1 site in human SP-B promoter |
| TTF-1mutant SP-B | 5′- GCACCAGGACGGCTCATCACAGCAA -3′ | mutant TTF-1 site in human SP-B promoter |
| HNF-3 SP-B | 5′- GCAAAGACAAACACTGAG -3′ | HNF-3 site in human SP-B promoter |
HNF, hepatocyte nuclear factor; SP, surfactant protein.