Fig. 3.

Bleo induces greater collagen deposition in the lungs of male mice. A: mouse lung sections stained with Masson's trichrome. Collagen deposition (blue) is observed adjacent to airways and large vessels in Sal control mice, as well as in fibrotic areas in response to Bleo. B: quantitative assessment of collagen deposition indicates male mice have a significantly greater percentage of lung collagen compared with female mice (*P < 0.05). There was no significant difference between groups of Sal-instilled mice by ANOVA (P > 0.05). AM mice have a higher percentage of collagen vs. YM mice (**P < 0.01). YM and YF mice and AM Bleo-instilled mice have significantly more collagen them Sal-treated mice (at least P < 0.05). Values are means ± SE.