Table 1.

Amino acid sequence of COOH-terminal region of human, mouse, and rat CXC chemokines

Chemokine	Amino Acid Sequence																							Reference
Human CXCL8	C	L	D	P	K	E	N	W	V	Q	R	V	V	E	K	F	L	K	R	A	E	N	S	11,*13
Rat MIP-2	C	L	N	P	E	A	P	L	V	Q	R	I	V	Q	K	I	L	N	K	G	K	A	N	17
Murine MIP-2	C	L	D	P	E	A	P	L	V	Q	K	I	I	Q	K	I	L	N	K	G	K	A	N	27
Rat CINC	C	L	D	P	E	A	P	M	V	Q	K	I	V	Q	K	M	L	K	G	V	P	K		30
Murine KC	C	L	D	P	E	A	P	L	V	Q	K	I	V	Q	K	M	L	K	G	V	P	K		1

The COOH-terminal regions of the chemokines have been aligned on the last cysteine; the positively charged amino acids arginine (R) and lysine (K) are in bold type; and the 4 amino acids of the GAG-binding domain found in the COOH-terminus of human CXCL8 are italicized. The glycosaminoglycan-binding domain of human CXCL8 consists of the 5 positively charged amino acids, K20 (not shown), R60, K64, K67, and R68.

MIP-2, macrophage inflammatory protein-2; CINC, cytokine-induced neutrophil chemoattractant; KC, keratinocyte-derived cytokine.

^*Mutations in K64 and R68 caused the largest decrease in heparin binding (11).