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. Author manuscript; available in PMC: 2011 Oct 12.
Published in final edited form as: Environ Mol Mutagen. 2009 Jan;50(1):10–26. doi: 10.1002/em.20433

TABLE I.

Oligonucleotide Primers for PCR Amplification and Vertical DGGE Analysis of the 22 Human tRNA Genes and Flanking Regions

PCR conditions
 PCR product
Oligonucleotide namea Primer sequence mMol MgCl2 PCR cycles Sequence position Length (bases) tRNA gene(s) amplified
H tRNA 1 Sense TGCTAACCCCATACCCCGAA 2.0 32 5′-528 193 tRNAPhe
H tRNA 1 Antisense PS ACTCACTGGAACGGGGATGCTT 3′-720
H tRNA 2 Sense PS TTAGTTGAACAGGGCCCTGAAGC 2.0 32 5′-1451 279 tRNAVal
H tRNA 2 Antisense AATGGTTTGGCTAAGGTTGTCTGG 3′-1729
H tRNA 3 Sense GTGCAGCCGCTATTAAAGGTTCGT 2.0 35 5′-3014 407 tRNALeu
H tRNA 3 Antisense PS GTTGGGGCCTTTGCGTAGTTGTAT 3′-3420
H tRNA 4 Sense ATTACAATCTCCAGCATTCCCCCT 2.2 30 5′-4231 329 tRNAIle, tRNAGln, tRNAMet
H tRNA 4 Antisense PS TCAGGTAAAAAATCAGTGCGAGCTT 3′-4559
H tRNA 7 Sense CCCTTACCACGCTACTCCTACC 1.5 32 5′-5461 254 tRNATrp, tRNAAla, tRNAAsn
H tRNA 7 Antisense PS TGATTAGGGTGCTTAGCTGTTAACT 3′-5714
H tRNA 8 Sense TTAAGCTAAGCCCTTACTAGACCAA 2.5 30 5′-5641 289 tRNACys, tRNATyr
H tRNA 8 Antisense PS GAGAATAGTCAACGGTCGGCGAA 3′-5929
H tRNA 12 Sense CATTCGAAGAACCCGTATACATAAAA 2.0 32 5′-7414 315 tRNASer, tRNAAsp
H tRNA 12 Antisense PS GTTGTGAGTGTTAGGAAAAGGGCA 3′-7728
H tRNA 13 Sense CCCATCGTCCTAGAATTAATTCCCC 2.0 30 5′-8207 277 tRNALys
H tRNA 13 Antisense PS TTGGTGAGGGAGGTAGGTGGTAGTTT 3′-8483
H tRNA 14 Sense CGCCTGATACTGGCATTTTGTAG 2.0 32 5′-9920 230 tRNAGly
H tRNA 14 Antisense PS TGTAGCCGTTGAGTTGTGGTAGTC 3′-10149
H tRNA 15 Sense AGCCCTAAGTCTGGCCTATGAGTGA 2.2 32 5′-10352 134 tRNAArg
H tRNA 15 Antisense PS TGTAAATGAGGGGCATTTGGT 3′-10485
H tRNA 16 Sense TATCCCCCATTCTCCTCCTATCCCT 2.0 32 5′-12081 419 tRNAHis, tRNASer, tRNALeu
H tRNA 16 Antisense PS TGAATATTGTTGTGGGGAAGAGACTG 3′-12499
H tRNA 17 Sense CGACCACACCGCTAACAATCAA 2.0 32 5′-14559 215 tRNAGlu
H tRNA 17 Antisense PS GGGGTTAATTTTGCGTATTGGGG 3′-14773
H tRNA 18 Sense PS CATCATTGGACAAGTAGCATCCGT 2.0 32 5′-15790 286 tRNAThr, tRNAPro
H tRNA 18 Antisense ATGGGTGAGTCAATACTTGGGTGG 3′-16075
a

The oligonucleotide denoted with PS has a psoralen derivative linked to the 5′ end.