Table 3.

Oligonucleotide primers used to amplify and sequence mouse and human Tlr4 genes

Mouse		Primate
Amplification
Exon 1	↑ CAGTCGGTCAGCAAACGCCTTCTTC	↑GCTCGGTAAACGGTGATAG
	↓CAAGGCAGGCTAGCAGGAAAGGGTG	↓TGAGAAGTTCTGGGCAGAAG
Exon 2	↑ TTATTCATCTTTGGAGAGGAGTGG	↑TCTCTGGTCTAGGAGAGG
	↓ AAGGAAGTTTAGTTAGAACCACCTTG	↓ CCAGTCCAATAATGAAATG
Exon 3	↑ TCTCCTGCTCACACCATCATCACCTG	↑ CCAGTCCAATAATGAAATG
	↓ CATGTGTTCCATGGGCTCTCGGTC	↓ TGACTTTCTTTGTCATGGGTTCCTTGACTG
Sequencing
Exon 1 same as above
Exon 2 same as above
Exon 3 primary	1↓ ATGCCATGCCTTGTCTTC	3.1 ↑ GAGCTGGATGACTAGGATTAATATTC
	2↓TTTAAATTCTCCCAAG	3.1 ↓TCAAATTGCACAGGCCCTCTAG
	3↓CAGCTCTTCTAGACC	3.2 ↑CAATCTCTCTTTAGACCTGTCC
	4↑ TGTGAACATCAGAAATTCCT	3.2 ↓AATACTTTAGGCTGGTTGTCCC
	5↑ TGAGATTGCTCAAACATGG	3.3 ↑ GAAGTTGATCTACCAAGCCTTG
	6↑ TTGAAACAATTGAAGACAAGGC	3.3 ↓ GGAAGTCATTATGTGATTGAGAC
	7↑ CCTGGCTGGTTTACACGTC	3.4↑ CTTCCTGGACCTCTCTCAGTGTCAAC
	8↑ TTTCATGGGTCTAGAAGAGCTG	3.4↓ GAAGGCAGAGCTGAAATGGAGG
	9↓ AAGAACTGCTTCTGTTCC	3.5↑ TCAGATGAATAAGACCATCATTGGTG
	10↓ TCAGAAACTGCCATGTTTG	3.5↓ AACAAGTGTTGGACCCAG
Exon 3 secondary	5'↑ TGAGCTGGTAAAGAATTTAG	1↑ GTAAATTTGGACAGTTTCC
	7'↑ CTGACGAACCTAGTACATGTG	2↑ TTCAGTATTCCTATCACTCAG
	9'↓ ATGTCAAGTTTGTTGTGTT	3↑ TTATAAGTGTCTGAACTCCC
		4↑ TCGGTCCTCAGTGTGCTTG
		5↑ GTGTCCCAGCACTTCATC
		6↑ AACCTCCTGAGGCATTTC
		7↓ GTTTCAAATTGGAATGCTG
		8↓ AAGGAAACGTATCCAATG
		9↓ AAGCACACTGAGGACCGAC
		10↓ GATGAAGTGCTGGGACAC
		11↓ TCCTCTCTAGATAGATGTTG
		12↓ TTTCTTTGTCATGGGTTC
		0↑ TTTAGGTTCTTATTCAGCAG
		0↓ GCTCTAGATTGGTCAGATTAG

↑ Primer matches + strand; ↓primer matches - strand.