Table I. AChBP ligand binding kinetics.

k₁ and k₋₁ were determined from rate measurements at various ligand concentrations according to k_obs = k₁[L] + k₋₁ (cf. Fig. 2) and K_d was calculated from k₋₁/k₁. S.E. values reflect values from three or more measurements. Where S.E. values are not shown, values reflect an average of two measurements.

Ligand	k1	k−1	Kd
	× 108 M−1 s−1	s −1	nM
Decamethonium	3.2 ± 0.17	120 ± 17	380
Dansyl-C2-cholinea	2.1	2.9	14
Gallamine	2.5 ± 0.14	36 ± 6.5	140
d-Tubocurarine	2.0	30	150
(+)-Epibatidine	1.7 ± 0.26	0.027 ± 0.037	0.16
(−)-Nicotine	1.5	5.7	38
Dansyl-C6-cholineb	1.3	7.6	58
Acetylcholine	1.1 ± 0.12	120 ± 16	1000
Waglerin-1	0.048	31	6500
α-Cobratoxin	0.033	0.011	3.2
α-Bungarotoxin	0.0097 ± 0.002	0.0017 ± 0.0003	1.8

^a5-Dimethylaminonaphthylsulfonamidoethyltrimethylammonium.

^b5-Dimethylaminonaphthylsulfonamidohexyltrimethylammonium.