Figure 2.

Average paw biting/licking values of different rat groups across different treatments in the formalin test. Data are expressed as mean (±standard error of the mean) time in seconds spent licking/biting the hind paw after injection of 5% formalin 60 μL. Significant differences were found between male rats that received Ro 25-6981 before formalin injection and male rats that received saline (A) at 0–10 minutes and 10–20 minutes (P < 0.05). Moreover, significant differences were also found between female rats that received Ro 25-6981 before formalin injection and female rats that received saline (B) at 0–10 minutes (P < 0.05). Significant differences were also found between male rats that received Ro 25-6981 before formalin injection and male rats that received saline (C) at 30–40 minutes (P < 0.05), and between male rats that received Ro 25-6981 after formalin injection and male rats that received saline (D) at 30–40 minutes (P < 0.05). All analyses were performed using analysis of variance tests.

Abbreviations: Male Ro pre-formalin, male rats that received Ro 25-6981 before formalin injection; male Ro post-formalin: male rats that received Ro 25-6981 after formalin injection; female Ro pre-formalin, female rats that received Ro 25-6981 before formalin injection; female Ro post-formalin, female rats that received Ro 25-6981 after formalin injection; male saline, male rats that received saline; female saline, female rats that received saline.