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. Author manuscript; available in PMC: 2011 Oct 12.
Published in final edited form as: Acta Neuropathol. 2007 Mar 20;113(5):501–511. doi: 10.1007/s00401-007-0207-8

Fig. 1.

Fig. 1

Microtubule assembly in Noco/Tri cells. 3T3 cells were extracted with 0.2% Triton X-100 and used as a “cellular platform” for MT assembly. a–c Microtubule network in intact 3T3 cells. d–f detergent extracted cells with or without pre-treatment with Nocodazole. MT structure is faithfully preserved in detergent extracted 3T3 cells (d). Pretreatment with Nocodazole destroys endogenous MT (e) and its components are largely lost on detergent extraction (f). Rat brain cytosol, at various concentrations (5, 10, 15, 25 or 50%) was added to Nocodazole pre-treated, detergent extracted 3T3 cells (Noco/Tri cells) in the presence of 1 mM GTP to study MT assembly (g–k). Nocodazole inhibited MT assembly when added to the rat brain cytosol on Noco/Tri cells (l). Alpha tubulin (DM1A, green), tyrosinated tubulin (YL1/2, red). Scale bar represents 25 μm