Fig. 4.

(A) Growth curves on a semilog scale based on the OD₆₄₀ values of isolates IST439, IST4103, IST4110, and IST4113 in iron-limited CDM (○) and in the same medium supplemented with 100 μM Fe³⁺ (•). (B) Growth curves based on the concentrations of viable cells (expressed as numbers of CFU per ml) in the cultures of isolates IST439 (• and ○) and IST4113 (■ and □) in the same iron-limited CDM (○ and □) and in this medium supplemented with 100 μM Fe³⁺ (• and ■). (C) Growth curves of IST439 (• and ○) and IST4113 (■ and □), compared on a linear scale, in CDM (○ and □) and in CDM supplemented with 100 μM Fe³⁺ (• and ■). (D) Culture optical densities after 24 h of growth in CDM or CDM supplemented with 100 μM Fe³⁺ for all sequential clonal variants of B. cenocepacia obtained during long-term colonization of patient J.