Fig. 5.

Anaerobiosis-induced transcriptional modulation of genes involved in QS regulation. (A) Quantitative RT-PCR analysis of genes that were previously determined to be directly regulated by LasR. Assay conditions were identical to those described in Fig. 4B. Three independent experiments were performed, and mean values ± SD are displayed in each bar. *, P < 0.01 versus transcript levels in PAO1 grown aerobically. (B) Downstream effects of suppressed transcription of mvfR on the expression of pqsA and pqsC as assessed by qRT-PCR. *, P < 0.01 versus transcript levels in PAO1 grown aerobically.