Table 1.
Oligonucleotide primersa
| Name | Sequence (5′ to 3′) |
|---|---|
| ppsAF | GCGAGGACCTGGTCGGTATC |
| ppsAR | GGCCTTGTTGAGGTTGGTC |
| ppsBF | GAACTCTGCCACGAGCTGG |
| ppsBR | GCACCGATGACGAGCTG |
| ppsDF | GTCTTAATTAAGGAAACCCTGGGACTCGAC |
| ppsDR | TAGGCGCGCCGCCAAGTGAATTGCCACCAG |
| ppsDF2 | CTGGAATTCTAAGAAGGAGATATACATATGACAAGTCTGGCGGAGC |
| ppsDR2 | CTGGTTAACTCGGGGATGCTCACAGGTC |
| ppsDR4 | AAGCTTGAGGGCGGATGTGAT |
| MVinsF | AGCGAGGACAACTTGAGC |
| ppsDF4 | CGATTCGCGCTCAGCTAGAC |
| pJGR | AAATGCCGATATCCTATTGGC |
| pJGF | GTGGACCTCGACGACCTC |
a
Restriction enzyme sites are underlined. The strong ribosome-binding site in primer ppsDF2 is indicated in italics.