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Substrate specificity of P. gingivalis mutants. (A) Table showing substrates used in this study with their respective glycoside linkages to the substrate. (B) Sialidase activity of P. gingivalis W83 and isogenic mutants FLL401, FLL402, and FLL403. The assay was performed using the Amplex Red neuraminidase (sialidase) assay kit (Molecular Probes). The three P. gingivalis mutants showed significant reduction in activity compared with the wild type (*, P < 0.001; **, P < 0.01).
