Table 1.
Strains and plasmids used
| Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
| Strains | ||
| P. gingivalis W83 | Wild type | 31 |
| FLL92 | vimA-defective mutant | 52 |
| FLL401 | PG0778 defective | This study |
| FLL402 | PG1724 defective | This study |
| FLL403 | PG0352 defective | This study |
| FLL401c | FLL401 with pFLL401a | This study |
| FLL402c | FLL402 with pFLL402a | This study |
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ thi-1 gyrA96 relA1 | Invitrogen |
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 ara139 Δ(ara-leu)7697 galU galK rpsl (Strr) endA1 nupG | Invitrogen |
| Plasmids | ||
| pCR-2.1.TOPO | Apr Kmr | Invitrogen |
| pVA2198 | SprermF-ermAM | 12 |
| pT-COW | AprtetQ | 13a |
| pFLL401a | AprtetQ TC::PG0778 | This study |
| pFLL402a | AprtetQ TC::PG1724 | This study |
| pFLL403c | AprtetQ TC::PG0352 | This study |
| pFLL403a | pCR-2.1-TOPO PG0352-PG0353 | This study |
| pFLL403b | pFLL403a with PG0352 interrupted with ermF-ermAM | This study |