Table 1.
List of primers used for the chicken cytokine mRNA quantification
| Primer | Sequence (5′→3′) | Source or reference |
|---|---|---|
| IL-βFor | GAAGTGCTTCGTGCTGGAGT | This study |
| IL-βRev | ACTGGCATCTGCCCAGTTC | |
| IL-8For | ATGAACGGCAAGCTTGGAGCT | 26 |
| IL-8Rev | GCAGCTCATTCCCCATCTT | |
| IL-12βFor | TGGTCCACGCTTTGCAGAT | 4 |
| IL-12βRev | AAGGTTAAGGCGTGGCTTCTTA | |
| IL-17For | TATCAGCAAACGCTCACTGG | This study |
| IL-17Rev | AGTTCACGCACCTGGAATG | |
| IL-18For | ACGTGGCAGCTTTTGAAGAT | 26 |
| IL-18Rev | GCGGTGGTTTTGTAACAGTG | |
| IL-22For | CAGACTCATCGGTCAGCAAA | This study |
| IL-22Rev | GGTACCTCTCCTTGGCCTCT | |
| IFNγFor | GCCGCACATCAAACACATATCT | 4 |
| IFNγRev | TGAGACTGGCTCCTTTTCCTT | |
| TNF-αFor | AATTTGCAGGCTGTTTCTGC | 26 |
| TNF-αRev | TATGAAGGTGGTGCAGATGG | |
| iNOSFor | GAACAGCCAGCTCATCCGATA | 4 |
| iNOSRev | CCCAAGCTCAATGCACAACTT | |
| Gal1For | GAAATGCTCAAGATTTTACCTCTG | This study |
| Gal1Rev | GAAGTTTCCAGAAGCGAGAAG | |
| Gal2For | AGGATTCTTTACCTGCTTTTCTCT | This study |
| Gal2Rev | AGCTTCCGACTTTGATTAGATG | |
| Gal4For | GTGTCTGTAGGTGGACAACATCT | This study |
| Gal4Rev | ATTGCATGTGATATCTTGGAGAAC | |
| Gal6For | TACTGGAGAAGGGAGACAGAAG | This study |
| Gal6Rev | TGATATCTACAAGCATGAATAGGG | |
| GAPDHFor | CCTGCATCTGCCCATTT | 6 |
| GAPDHRev | GGCACGCCATCACTATC | |
| TBPFor | TAGCCCGATGATGCCGTAT | 13 |
| TBPRev | GTTCCCTGTGTCGCTTGC | |
| UBFor | GGGATGCAGATCTTCGTGAAA | 6 |
| UBRev | CTTGCCAGCAAAGATCAACCTT |