Fig. 2.
Granulocyte and macrophage kinetics in neonatal sepsis. Spleen, blood, bone marrow, and peritoneal exudates were collected from neonates at different time points after sepsis (LD40). Tissue cell suspensions were then stained for granulocytes (Gr-1+ CD11b+) (A and C) and macrophages (F4/80+ CD11b+) (B and D) and analyzed via flow cytometry. Panels A and B represent the percentage of total live cells in the blood, while panels C and D show total cell number in the spleen, bone marrow, and peritoneum (n = 4 per group per time point; values represent the means ± SDs; *, Tukey's one-way ANOVA, P < 0.01).