Fig. 6.

Recombinant CXCL10 improves phagocytosis in granulocytes and macrophages. Neonatal splenocytes isolated via density gradient were incubated with or without murine CXCL10 (100 ng/ml) for 4 h. Cells were then washed and incubated with FITC-labeled beads and stained for granulocyte (Gr-1⁺ CD11b⁺) (A) and macrophage (F4/80⁺ CD11b⁺) (B) cell surface markers. Cells were first gated on granulocyte or macrophage populations, and then FITC⁺ cells were considered phagocytic. The figure represents the summary of three experiments, and histograms are representative samples from one of the experiments (values represent the mean ± SDs; *, Student's t test, P < 0.05).