Fig. 7.

Inhibition of CXCL10 reduces phagocytic function of granulocytes and macrophages in vivo. Two hours prior to sepsis (LD₄₀), neonates were administered either anti-CXCL10 or control IgG (0.5 mg) i.p. Twelve hours following sepsis (LD₄₀), peritoneal washes were harvested and incubated with FITC-labeled latex beads and subsequently stained for granulocyte (Gr-1⁺ CD11b⁺) and macrophage (F4/80⁺ CD11b⁺) cell surface markers. Cells were first gated on granulocyte or macrophage populations, and then FITC⁺ cells were considered phagocytic. Histograms of representative samples (A) and the summary of two experiments for both granulocytes (B) and macrophages (C) are presented (n = 6 per group; values represent the mean ± SDs; *, Student's t test, P < 0.05).