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T cell polarization by LOS-triggered DCs. LOS-matured DCs with 10 ng/ml of LPS, GB11, the Cst-II mutant, and GB16 (A) or DCs matured with 10 ng/ml LPS, GB2, GB2 Cst-II KO, GB19, and GB19 sialidase (B) were incubated with naïve T cells. After 14 days, the intracellular IL-4 and IFN-γ production by T cells was measured by FACS analysis. Average percentages of positive cells for two donors are shown.
