Fig. 5.

(A) Activation of MAPKs in the lung following E. coli infection. Total proteins in the lung were isolated from MCP-1^−/− and control mice at 6 and 24 h after infection with E. coli and resolved on an SDS-polyacrylamide gel, and the membrane was blotted with the Abs against the activated/phosphorylated (encircled P) form of MAPKs as described in Materials and Methods. This is a representative of 3 separate experiments with identical results. (B) Densitometric analysis of MAPK activation was performed from 3 separate blots. *, P < 0.05 for difference between MCP-1^−/− mice and their WT controls.