Table 1.
Cytokine and NO2− levels in SNs of BM-Mφ cultures and BM-Mφ/NK coculturesa
| Culture no. | BM-Mφ | Cultured with: |
Measured concn (±SEM)b |
||||
|---|---|---|---|---|---|---|---|
| NK | Stimulus | Blocking agent | IFN-γ (ng/ml) | TNF (pg/ml) | NO2− (μM) | ||
| 1 | WT | 0 (±0) | 6 (±3) | 0 (±0) | |||
| 2 | WT | IFN-γ + TNF | 12 (±4) | 1,750 (± 512) | 56 (±3) | ||
| 3 | WT | IL-12 + IL-18 | 0 (±0) | 18 (±3) | 3 (±3) | ||
| 4 | WT | WT | 0 (±0) | 69 (±29) | 0 (±0) | ||
| 5 | WT | WT | IL-12 + IL-18 | 144 (±43) | 274 (±67) | 50 (±4) | |
| 6 | IFN-γR−/− | 0 (±0) | 4 (±1) | 0 (±0) | |||
| 7 | IFN-γR−/− | IFN-γ + TNF | 22 (±4) | 652 (±131) | 0 (±0) | ||
| 8 | IFN-γR−/− | IL-12 + IL-18 | 0 (±0) | 24 (±18) | 0 (±0) | ||
| 9 | IFN-γR−/− | WT | 0 (±0) | 76 (±51) | 0 (±0) | ||
| 10 | IFN-γR−/− | WT | IL-12 + IL-18 | 276 (±43) | 241 (±62) | 0 (±0) | |
| 11 | WT | IgG | 0 (±0) | 5 (±4) | 0 (±0) | ||
| 12 | WT | Anti-TNF | 0 (±0) | 1 (±0) | 0 (±0) | ||
| 13 | WT | IFN-γ + TNF | IgG | 13 (±3) | 801 (±356) | 48 (±3) | |
| 14 | WT | IFN-γ + TNF | Anti-TNF | 6 (±3) | 221 (±140) | 4 (±2) | |
| 15 | WT | IL-12 + IL-18 | IgG | 0 (±0) | 13 (±4) | 0 (±0) | |
| 16 | WT | IL-12 + IL-18 | Anti-TNF | 0 (±0) | 8 (±6) | 0 (±0) | |
| 17 | WT | WT | IgG | 0 (±0) | 110 (±89) | 0 (±0) | |
| 18 | WT | WT | Anti-TNF | 0 (±0) | 50 (±37) | 0 (±0) | |
| 19 | WT | WT | IL-12 + IL-18 | IgG | 106 (±75) | 168 (±81) | 52 (±5) |
| 20 | WT | WT | IL-12 + IL-18 | Anti-TNF | 116 (±72) | 67 (±35) | 49 (±13) |
a
BM-Mφ from B6 or IFN-γR−/− mice were infected for 18 h with a 10-fold excess of L. infantum promastigotes and subsequently cultured for 72 h with different cytokines with or without addition of splenic NK cells from B6 mice. Under some conditions, anti-TNF blocking antibody or a control IgG was added. SNs were tested for cytokine and NO2− content.
b
Data are expressed as mean (±SEM) of 3 to 6 independent experiments.