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. 2011 Jul;79(7):2608–2618. doi: 10.1128/IAI.01219-10

Fig. 2.

Fig. 2.

GFP expression controlled by PviuB in vivo in zebrafish. (A) GFP expression levels of BL21(DE3)/pUTG (gray bars) and BL21(DE3)/pUTBG (black bars) incubated in immersion solution. BL21(DE3)/pUTBG (white bars) was induced to synthesize GFP as a positive control. Immersion solution was prepared as phosphate-buffered saline (PBS) with 1% bovine serum albumin. (B) Visualization of GFP synthesis under the control of PviuB in zebrafish by fluorescence microscopy. Fish were immersed in bacterial suspensions (108 CFU/ml) of BL21(DE3)/pUTG (panel I) and BL21(DE3)/pUTBG (panel II) for 1 h and detected at 9 h postinfection. For further analysis, zebrafish infected by BL21(DE3)/pUTBG were observed by confocal microscopy. Panels III, IV, and V, different parts of the fish from head to tail, respectively (magnification, ×400); panel VI, details of the site marked by a box in panel IV (magnification, ×1,000).