Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Bacteria | ||
| E. coli | ||
| Top10F′ | General cloning strain | Invitrogen |
| H1717 | Indicator strain; fhuF::λplacMu | 58 |
| BL21(DE3) | F−ompThsdSgal; expression host, vaccine delivery vector | Invitrogen |
| DH5α | recA1endA1gyrA96thi1hsdR17supE44relA1lacZΔM15; gene source of iron-regulated promoters | Invitrogen |
| V. anguillarum MVM425 | Wile-type, Pacific Ocean prototype (O1 serotype) fish pathogen; gene source of iron-regulated promoters | Our lab |
| V. parahaemolyticus | Wild-type pathogen from fish; gene source of iron-regulated promoters | Our lab |
| A. hydrophila | Wild-type pathogen from fish; gene source of gapA | Our lab |
| Genomes and plasmids | ||
| V. cholerae chromosome I | O1 biovar El Tor strain N1696; gene source of iron-regulated promoters | Biao Kan |
| pEIB1 | Endogenous plasmid of V. anguillarum MVM425; gene source of iron-regulated promoters | Our lab |
| pBV220 | Prokaryotic expression vector; source of rrnBT1T2 terminator and MCS | Zhiqing Zhang |
| mTn5gusA-pgfp12 | Carrying the gfpuv gene; gene source of gfp | Chuanwu Xi |
| φX174 genome | E gene source | NEB |
| pMD19T-simple | Cloning vector; Ampr | Takara |
| pMD19TB | pMD19T-simple derivative; PviuB insertion | This study |
| pUC18 | Cloning vector; Ampr | Takara |
| pET28a | Expression vector; Kmr | Novagen |
| pUT | Promoter-screening vector; pUC18 derivative with lac promoter and MCS deletion and with rrnBT1T2 terminator and MCS from pBV220 insertion | This study |
| pUTG | pUT derivative containing gfp open reading frame | This study |
| pUTBG | pUT derivative containing PviuBgfp TT | This study |
| pUTa | pUT derivative; replacement of pBR322 ori with p15A ori | This study |
| pUTaBE | pUTa derivative containing PviuaBE TT | This study |
| pETGA | pET28a derivative containing PT7gapA TT | This study |