Fig. 7.
Prior AHL treatment of murine macrophages induced bacterial phagocytosis. RAW 264.7 murine macrophages were treated with 200 μM C4-HSL, C6-HSL, C4-HSL and C6-HSL together, or vehicle for 1 h. Macrophages were infected with A. hydrophila at an MOI of 10, and after extracellular bacteria were killed by gentamicin treatment, host cells were lysed and the intracellular bacteria on the LB agar plates were counted. Data from at least three independent experiments (each treatment in triplicate) were averaged, and the standard deviations are shown. Asterisks describe statistically significant differences in phagocytosis between AHL-treated and vehicle-treated control murine macrophages.