Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant properties | Reference or source |
|---|---|---|
| Strains | ||
| E. faecalis | ||
| V19 | Strain V583 (clinical isolate), without plasmid | 38 |
| V19 ΔslyA | V19 isogenic derivative slyA mutant | This study |
| V19 ΔslyA pCU1-slyA | V19 isogenic derivative slyA mutant with pCU1 carrying slyA | This study |
| E. coli | ||
| XL1Blue | recA1 endA1 gyrA96 thi-1 hsdR17supE44 relA1 lac[F′ proABlacIq ZΔM15] Tn10 (Tetr) | Stratagene |
| DH5α | recA endA1 gyrA96 thi-1 hsdR17 [rK− mK+supE44 relA1 lacZΔM15] | 15 |
| Plasmids | ||
| pMad | oripE194ts, Emr, Ampr, bgaB | 1a |
| pMad-ΔslyA | pMad carrying slyA deletion | This study |
| pCU1 | Cmr shuttle vector for E. coli and E. faecalis | 2 |
| pCU1-slyA | pCU1 carrying slyA | This study |