Strains |
|
|
E. faecalis
|
|
|
V19 |
Strain V583 (clinical isolate), without plasmid |
38 |
V19 ΔslyA
|
V19 isogenic derivative slyA mutant |
This study |
V19 ΔslyA pCU1-slyA
|
V19 isogenic derivative slyA mutant with pCU1 carrying slyA
|
This study |
E. coli
|
|
|
XL1Blue |
recA1 endA1 gyrA96 thi-1 hsdR17supE44 relA1 lac[F′ proABlacIq ZΔM15] Tn10 (Tetr) |
Stratagene |
DH5α |
recA endA1 gyrA96 thi-1 hsdR17 [rK− mK+supE44 relA1 lacZΔM15] |
15 |
Plasmids |
|
|
pMad |
oripE194ts, Emr, Ampr, bgaB
|
1a |
pMad-ΔslyA
|
pMad carrying slyA deletion |
This study |
pCU1
|
Cmr shuttle vector for E. coli and E. faecalis
|
2 |
pCU1-slyA
|
pCU1 carrying slyA
|
This study |