Fig. 11.

Deletion of sopB abolishes the differences in cecal inflammation in Salmonella-infected WT and Akt2 KO mice. Mice defective in Akt2 and WT mice were treated with streptomycin prior to infection with the sopB deletion mutant ΔsopB and sacrificed at 1 day postinfection. Representative H&E staining of ceca from one mouse from each group of at least 3 mice is shown. At 1 day postinfection, severe inflammation in the lumen of the ceca of sopB-infected WT mice (A) and Akt2 KO mice (B) was observed. Images are shown at magnifications of ×50 and ×200. (C) Pathological scoring for the cecal samples harvested from WT mice and Akt2 KO mice at 1 day postinfection with the sopB deletion mutant ΔsopB and the Salmonella WT SL1344 is shown. P values for the differences in pathological scoring of the ceca among the various groups were determined by two-tailed, unpaired t test.