Fig. 7.

S. Typhimurium infection is associated with an elevated influx of CD11b⁺ F4/80⁺ macrophages in the colons and ceca of mice lacking Akt2. Mice deficient in Akt2 and WT mice were treated with streptomycin prior to infection with S. Typhimurium and sacrificed at 1 day postinfection. Single-cell suspensions from the colons and ceca of uninfected WT (A) and Akt2 KO (B) mice and infected WT (C) and Akt2 KO (D) mice were subjected to FACS staining. Representative FACS plots comparing the expression levels of CD11b and F4/80 macrophages harvested from the colons and ceca of each group are shown. FACS plots were gated on live cells, with 7-AAD-positive dead cells excluded from analysis. Numbers indicate the percentage of cells in each quadrant. FACS plots are representative of four independent experiments with a total of 12 mice from each group.