Table 1.
Crystal structure Tyr to Trp distances and calculated energy transfer efficiencies
Acceptor | Donor | κ2 | Ro | R | E |
---|---|---|---|---|---|
W10 | Y26 | 0.199 | 12.5 | 27.1 | 0.009 |
Y43 | 0.025 | 8.8 | 33.1 | 0.0004 | |
Y57 | 0.282 | 13.2 | 12.4 | 0.597 | |
Y64 | 0.070 | 10.5 | 18.8 | 0.029 | |
W12 | Y26 | 0.536 | 14.7 | 22.8 | 0.068 |
Y43 | 2.474 | 19.0 | 29.4 | 0.068 | |
Y57 | 0.029 | 9.0 | 10.9 | 0.248 | |
Y64 | 0.084 | 10.8 | 21.9 | 0.014 |
Distances, R, (Å) measured between center of Tyr ring and midpoint of bond between Trp Cδ2 and Cε2 (see Fig. 1). Dipole orientation factor, κ2, Förester critical distance, Ro, and energy transfer efficiency, E, calculated from equations (2) and (3) as described in text.