(A) Far-ultraviolet spectra were collected at 0.2 mg/ml in a 1 mm cuvette at 22 °C, and after heating to 80 °C, recycling to 22 °C, returning to 95 °C, and back again to 22 °C. (B) Time course analysis at 220 nm, with temperature varying at 2 °C/min. Data are representative of trials from 2 independent Skp1 preparations.