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. 2011 Oct 12;6(10):e25397. doi: 10.1371/journal.pone.0025397

Figure 4. Stability of ubiquitin-IκBα fusion protein.

Figure 4

A) Ubiquitin-IκBα fusion was generated with an N-terminal HA and C-terminal V5 epitopes. B) HEK293 cells transfected with IκBα WT and ubiquitin-IκBα fusion were treated with 50 µg/ml CHX for the indicated times. The graph corresponds to the mean of three independent experiments. C) IκBα WT and ubiquitin-IκBα fusion were expressed in HEK293 and stimulated for 20 minutes with 10 ng/ml of TNFα. D) IκBα WT and ubiquitin- IκBα fusionwere expressed in HEK293 cells and stimulated with 10 ng/ml of TNFα for the indicated times.