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A) Effect of purine nucleotides on ppAGUAA-FAM and AGUAA-FAM RNA binding. KD values for ppAGUAA binding to wild-type dengue capping enzyme were determined in the presence of increasing concentrations of the indicated nucleotide. AGUAA binding was determined only in the presence of 50 µM GTP or Mock. B) Effect of SAM and SAH on ppAGUAA-FAM RNA binding affinity. n = 3.
