Table 1. Primers used for PCR detection of genes encoding virulence factors.
| Oligonucleotides sequences | size pb/PCR set | accession n° |
| edinA 1: 5′-TCATAGAAGTATCTAATACTTCTTTAGCA-3′ | 604/2 | M63917 |
| edinA 2 : 5′-TCCAACACGGTATTCTGTGCCTCTAGGTA-3′ | ||
| edinb 1: 5′-ACAGACTTAGTTGAAGCTACTAAATG-3′ | 522/5 | AB057421 |
| edinb 2: 5′-TGTCCCTGTAGGCAAAAGAACTTCTTG-3′ | ||
| edinC 1: 5′-AGGTCTTCCAGCTAATGCAGCTCCTT-3′ | 543/6 | NC 003265 |
| edinC 2: 5′-ACAGTTCAAAAGACAAAGAAGCTATT-3′ | ||
| etd 1: 5′-AATACATATGAAGAATCTGAAATTTTA3′ | 800/4 | AB057421 |
| etd 2: 5′-AAGTTATTCCATAATGATTAGAATGA-3′ | ||
| lukE-lukD 1: 5′-AATACTAATATTGAAAATATTGGTGATGG-3′ | 1882/8 | Y13225 |
| lukE-lukD 2: 5′-CTTAAATTTCGGGTTTAACTCCTCTATTTC-3′ | ||
| see 1: 5′-CTTACCGCCAAAGCTGTCG-3′ | 159/3 | M21319 |
| see 2: 5′-GTCCACTTGTAAATGGTAGCGAGAA-3′ | ||
| seg 1: 5′-AATTATGTGAATGCTCAACCCGAT-3′ | 408/3 | AF064773 |
| seg 2: 5′-CTTTAGTGAGCCAGTGTCTTGCTTTG-3′ | ||
| seh 1: 5′-CATCTACCCAAACATTAGCACC-3′ | 222/5 | U11702 |
| seh 2: 5-TAGAAATCAAGGTGATAGTGGCAA-3′ | ||
| sek 1: 5′-TGATACTCCTATAGCTAATCAACTACA-3′ | 300/3 | U93688 |
| sek 2: 5-ACATCAATCTCTTGAGCGGTAACA-3′ | ||
| sel 1: 5′-ACCAGAATCACACCGCTTAGAATAC-3′ | 422/2 | AF217235 |
| sel 2: 5′-TGGAATACTACACTCCCCTTATCAAAAG-3′ | ||
| set1-1: 5′-GAAGGTCTACAAGGCCAAAATGTCT-3′ | 363/6 | BX571856 |
| set1-2: 5′-TCAACACATCGCCCATGCGCTCGA-3′ | ||
| bbp 1: 5′-CGGCTAGTGATAATAAAGAAGTAGTG-3′ | 550/1 | BA000018 |
| bbp 2: 5′-CTTGTTGGAGCTGTAGCAACTGGTTT-3′ | ||
| clfB 1: 5′-ATTAGTGCAAACACAAACAGTGCG-3′ | 305/4 | AJ224764 |
| clfB 2: 5′-AGTTCCTTGCGCATTGGAAATCGT-3′ | ||
| cna 1: 5′-CGGGAGATATGCTACCAGAAGATA-3′ | 278/1 | BX571857 |
| cna 2: 5′-ATAGCCTTGTGGAATTGTTACATCA-3′ | ||
| ebp 1: 5′-AGACCAATCAGAATTAGAACATCA-3′ | 378/4 | U48826 |
| ebp2: 5′-TCAGAAACTGTTGAATGCTCAGTGT-3′ | ||
| fib 1: 5′-AGCGGCAATAGGTATTACTACAACT3′ | 220/2 | X72013 |
| fib 2: 5′-CGAATGTACCATCGTTAAATTCAT-3′ | ||
| fnbA 1: 5′-TTAACTTGGGATAATGGTTTAGTTT-3′ | 273/7 | AJ629521 |
| fnbA 2: 5′-GCTGATGAATCCGTTTCTTCTATTG-3′ | ||
| fnbB 1: 5′-TGGAAGAAACTAAAGCGACAGGTAC-3′ | 317/1 | AJ629502 |
| fnbB 2: 5′-TTCTTTAAACGTATATCTAACTTTTC-3′ | ||
| lbp 1: 5′-TGGTGTATATGACTACAGTAAGTT-3′ | 410/5 | AF065394 |
| lbp 2: 5′-CGTTTGTAGCAACAGCAATATCAGC-3′ |
Other virulence factors have been detected by multiplex PCR onto purified total DNA (Qiagen). Presence of genes encoding enterotoxins E, G, H, K, L, T, epidermolysin D (Etd), genes encoding Edin A and EDIN B and the genes encoding seven adhsesion factors (Cna, FnbA, FnbB Bbp, Clfb, Fib, Ebp and Lbp) was checked in 8 set in function of base size.