Fig. 6.

PDGFRα⁺-IM are closely associated with nNOS⁺ nerve fibers in the WT and W/W^v mouse IAS. Dual-labeling of PDGFRα⁺ cells (red) and nNOS⁺ nerve fibers (green) in whole-mount preparations within the IAS. PDGFRα⁺ cells form a network along the submucosal (SM, a, d) and myenteric (My, c,f) surfaces but these cells do not appear to be highly associated with nNOS⁺ nerve fibers. In contrast, intramuscular (IM) nNOS⁺ nerve fibers are closely aligned with PDGFRα⁺ cells (b, e). g Higher magnification image showing varicose nNOS⁺ nerve fibers closely aligned with PDGFRα⁺-IM. Optical section thicknesses: a 2 µm, b 2.5 µm, c 4 µm, d 2 µm, e 1.5 µm, f 3.5 µm, g 1.5 µm