Table 1.

Primary antibodies

Primary antibody	Source	Monoclonal or polyclonal	Host	Antigen	Specificity	Working dilution
Anti-mSCFR	R&D Systems, Minneapolis, Minn., USA	Poly	Goat	Extracellular domain of stem cell factor receptor	Specifically recognizes mouse SCFR with Western blot and ELISA	1:1000
Anti-KIT (ACK2)	Gibco BRL, Gaithersburg, Md., USA	Mono	Rat	Extracellular domain of the c-kit proto-oncogene	Recognizes two bands at 120 and 160 kDa corresponding to KIT in murine mast cell extract with Western blot	1:1000
Anti-PDGFRα	eBioscience, San Diego, Calif. USA	Mono	Rat	α Chain of the platelet-derived growth factor receptor	Recognizes mouse CD140a, the α chain of the platelet-derived growth factor receptor	1:500
Anti-PDGFRα	R&D Systems	Poly	Goat	Extracellular domain of mouse platelet-derived growth factor receptor α	Neutralizes mouse PDGFRα bioactivity	1:1000
Anti-nNOS	Gift from Dr. Piers Emson, Mol. Sci. Group, Cambridge, UK	Poly	Sheep	Neuronal isoform of nitric oxide synthase (nNOS)	Detects neuronal isoform of nitric oxide synthase (nNOS) in central and peripheral nitrergic neurons	1:1000
Anti-NOS1 (R-20)	Santa Cruz Biotechnology, Santa Cruz, Calif., USA	Poly	Rabbit	Epitope near the c-terminus of NOS1	Recognizes NOS1 (ncNOS)	1:500