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. 2011 Oct 12;6(10):e26080. doi: 10.1371/journal.pone.0026080

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Faggioli et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B). The plots summarize the percentage of mononucleated (A) and binucleated cells (B) classified according to the number of centrosomes found by co-immunostaining with anti γ-tubulin and anti-pericentrin. In this plotting the percentage of cells with 4 centrosomes is kept separate from cells carrying 1 and 2 centrosomes since, depending upon the DNA content, cells with 4 centrosomes could be classified as normal or abnormal. (C) Examples of normal and extranumerary centrosomes detected by colocalization of γ-tubulin (green) and pericentrin (red) in mono (top) and binucleated (bottom) hepatocytes for the different ages analyzed. For the older mice analyzed some example of extra-centrosomes are reported.