Figure 5. Characterization of peptide processing and MHC class I presentation.

Comparison of (A) Major Histocompatibility Complex (MHC) binding score, (B) proteasome cleavage score, (C) transporter associated with antigen processing (TAP) score, (D) processing score, which combines proteasomal cleavage and TAP transport scores, (E) total epitope score, and (F) MHC IC₅₀, in nM. The total epitope score is a summary of the proteasomal, TAP and MHC binding scores and between reactive peptide variants (red) and their corresponding non-reactive variants (purple). Peptides binding to HLA class I A and B alleles had their total processing scores and MHC IC₅₀ predicted using the Immune Epitope Database (www.immuneepitope.org), and scores were compared using the non-parametric Mann-Whitney test.