Figure 5. IP-10 Neutralization Inhibits the Migration of T Cells.

(A) SNU-638-Pml siRNA cells were grown in the absence or presence of IFN-γ (10 ng/ml) for 8 h, and supernatant was collected and pretreated with IP-10 neutralizing antibodies (5 µg/ml) or IgG (5 µg/ml) for 1 h prior to initiation of the transwell migration assay. The number of migrating Jurkat cells obtained from the lower chamber was analyzed by fluorescent count-bead mediated counting on a flow cytometer. (B) Transwell migration of EL-4 cells to culture supernatants from NIH3T3 cells transiently transfected with Pml siRNA in the presence of neutralizing IP-10 antibody or IgG control. Relative cell migration was determined by the number of the migrated cells divided by the number of cells migrating to supernatants without IFN-γ, and the value from parental cells was arbitrarily set at 1. Data shown are representative of at least three experiments. Bars, SD. *P<0.05.