Table 1. PCR primers used to amplify and sequence the DNA barcoding regions CO1 and ND1-16S.
| PRIMER | PRIMER SEQUENCE | DIRECTION |
| CO1 | ||
| LCO1490 | GGTCAACAAATCATAAAGATATTGG | Forward |
| LCO1490aphonopelma | TTTCTACTAATCACAAGGATATYGG | Forward |
| LCO1490cg | TTTCTACTAATCACAAGGATATTGG | Forward |
| LCO1490chalcodes | TTTCCACAAACCACAGGGATATCGG | Forward |
| LCO1490reversum | TTTCTACTAATCATAGGGATATTGG | Forward |
| C1-J-1751“SPID” | GAGCTCCTGATATAGCTTTTCC | Forward |
| C1-N-2776 | GGATAATCAGAATATCGTCGAGG | Reverse |
| ND1-16S | ||
| N1-J-12261 | TCRTAAGAAATTATTTGA | Forward |
| LR-N-13398 (16Sar) | CGCCTGTTTAACAAAAACAT | Reverse |
| LR-N-12945 | CGACCTCGATGTTGAATTAA | Internal reverse |