Figure 4. Shot is essential for photoreceptor morphogenesis in the mid-stage developing pupal eyes.

(A and B) Mid-stage pupal eyes (45% pd) with shot³ null mutant clones marked by the absence of the GFP and dotted lines (green, A' and B'). Crb (red, A, arrow) was almost absent, and E-cad (blue, A, arrowhead) was mislocalized from the apical center toward the surrounded distal areas (arrow, A). Stable microtubules (Acetub, red) were also mislocalized in the shot³ mutants (arrow, B).