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. Author manuscript; available in PMC: 2012 Oct 19.
Published in final edited form as: J Am Chem Soc. 2011 Sep 22;133(41):16642–16649. doi: 10.1021/ja207086x

Table 2.

Mismatch discrimination properties of FHNA.

Modification Tm (ΔTm = Tm(mismatch)–Tm (match)) (°C)a,b
X = A X = G X = C X = T
DNA 45.6 41.5 (−4.1) 32.4 (−13.4) 31.9 (−13.7)
FHNA 46.2 36.3 (−9.9) 34.3 (−11.9) 38.9 (−7.3)
LNA 50.8 45.6 (−5.2) 34.1 (−16.7) 37.2 (−13.6)
a

Sequence used for evaluation 5′-d(GCGTTtTTTGCT)-3′ where the bold letter indicates the modified nucleotide

b

Sequence of RNA complement 5′-r(AGCAAAAXAACGC)-3′ where X indicates the mismatch site.