Table 1. Behavior of somite cells from embryos lacking Myo/Nog cells in vitro.

Stage X–XII embryos treated with the G8 MAb and complement were grown for two days. Right and left somites were removed from ablated (Abl) and untreated (Untx) embryos. Some somite cells from ablated embryos were cultured with a piece of the neural tube (NT) and notochord (NC) dissected from untreated embryos. Cells were stained with antibodies to G8, muscle proteins and noggin on the fifth day in culture. A minimum of 400 cells were scored per culture. Values are the mean ± standard deviation of the percent of the total number of cells that were stained with antibody. The number of cultures scored is indicated in parentheses. With the exception of Wnt3a, statistically significant differences were found between untreated and ablated cultures with or without the neural tube and notochord (MyoD, myosin, 12101, GATA4 and CTpI: p < 0.0001; Pax3, G8, and noggin: p ≤ 0.07, 0.02, 0.01, respectively).

	Untx	Abl	Abl + NT/NC
Pax3	22 ± 10 (12)	37 ± 17 (8)	39 ± 21 (5)
MyoD	22 ± 12 (14)	2 ± 4 (13)	6 ± 5 (5)
Myosin	65 ± 15 (13)	18 ± 17 (13)	4 ± 8 (5)
12101	42 ± 18 (11)	1 ± 2 (9)	4 ± 6 (5)
G8	55 ± 17 (4)	2 ± 3 (4)	0.2 ± 0.1 (5)
Wnt3a	22 ± 21 (12)	31 ± 17 (8)	19 ± 3 (5)
Noggin	31 ± 24 (5)	0.5 ± 0.4 (4)	0.4 ± 0.6 (4)
GATA4	1 ± 1 (14)	50 ± 17 (16)	50 ± 10 (4)
CTpI	0.3 ± 0.4 (14)	18 ± 11 (15)	13 ± 7 (4)