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Schematics of lipopeptides used in EPL to generate lipidated semisynthetic proteins. A) Mono- and digeranylgeranylated peptides. B) S-farnesylated peptides modified with a cleavable thioester-linked palmitate (left) or noncleavable thioether-linked hexadecanoate (right) for maleimidocaproyl (MIC) ligation. C) Cys-Ser-Cys peptide modified with farnesyl-NBD (nitrobenzoxadiazole).
