Fig. S3.

Expressions of genes (measured using qRT-PCR) and activities of kinases (assessed by densytometric analysis of western-blots) involved in cell-cycle regulation in fibroblasts isolated from mice of different HO-1 genotype and cultured in vitro. Cells were stimulated with DMBA (5 μM) for 72 h or transduced with retroviral vectors harboring c-myc and then cultured for 72 h. A – p21, B – cyclin-D1, C – cyclin-D2, D – cyclin-D3, E – phosphorylated Akt, F – phosphorylated S6. G – representative blots showing p-Akt and p-S6: c – control; d – cells treated with DMBA; m – cells overexpressing c-myc. EF2 and eIF4E were used as a constitutive housekeeping genes and loading controls in qTY-PCR and western-blotting, respectively (* – P < 0.05 vs. HO-1^+/+, # - P < 0.05 vs. control, unstimulated). Each bar represents mean + SE.