Skip to main content
. Author manuscript; available in PMC: 2012 Oct 12.
Published in final edited form as: Chem Rev. 2011 Jun 23;111(10):6022–6063. doi: 10.1021/cr200075y

Fig. 32.

Fig. 32

(A) Competitive ABPP. The potency and selectivity of a single inhibitor (green square) can be evaluated against SHs in a native tissue proteome by chasing the inhibitor with a SH-directed activity probe (red circle) and visualization by in-gel fluorescence following SDS-PAGE separation. Inhibitor targets are detected as bands with reduced fluorescence. (B) Fluopol-ABPP. A single SH can be evaluated against many compounds by chasing inhibitors (yellow hexagon or green triangle) with a SH-directed activity probe (red circle). Active (green triangle) or inactive (yellow hexagon) inhibitors will produce low or high fluorescence polarization signals, respectively.